Lay summary
Although the impact of diet on health and disease is unquestionable, most of what we know about habitual diet is derived from self-reported dietary intakes. Reliance on only self-reported information is a fundamental limitation as we know that dietary intakes may be under or over reported at different rates in population subgroups, and to a varying extent across the life span. The proposed project seeks to address this limitation through the development and validation of a mass spectrometry based method to measure multiple metabolites which reflect dietary intakes. The project will work with already identified biomarkers of whole grains, vegetables, fruits, fish, and meats given the role of these food groups in the development of a broad range of non-communicable disease outcomes. Method development will be to measure multiple markers from the same plasma or urine sample, validation will be of true, actual, and habitual intakes in clinical and non-clinical